The Effects of Ischemic Preconditioning and K(ATP) channel Activation on the Expression of the PKC-epsilon, NF-kappaB and AP-1 in Ischemia-reperfused Rat Heart.
10.11637/kjpa.2006.19.3.165
- Author:
Dong Choon AHN
1
;
Seung Ha CHUN
;
Youn Kyoung SEO
;
Su Kyoung JEON
;
Hyun Joo PARK
;
Sang Wan LEE
;
Jeong Ha SIM
;
Doo Jin PAIK
Author Information
1. Department of Anatomy and Cell Biology, College of Medicine, Hanyang University, Korea. paikdj@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Ischemic peconditioning;
Rat heart;
PKC-epsilon;
NF-kappaB;
AP-1
- MeSH:
Animals;
Blotting, Western;
Coronary Vessels;
Glyburide;
Heart*;
Humans;
Immunohistochemistry;
Ischemia;
Ischemic Preconditioning*;
Ligation;
Male;
NF-kappa B*;
Pinacidil;
Rats*;
Reperfusion;
Transcription Factor AP-1*
- From:Korean Journal of Physical Anthropology
2006;19(3):165-178
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was aimed to elucidate the effects of K(ATP) activation during IPC on the PKC-epsilon, NF-kappaB and AP-1 in ischemia-reperfused rat hearts. SD male rats weighting from 300 to 350 g were split into 9 groups, such as sham control (S), IPC, 3 cycles of 5 min ischemia and 5 min reperfusion, continuous preconditioning (CP), 8 cycles of 5 min ischemia and 5 min reperfusion, K(ATP) opening (KO) with pinacidil (1.0 mg/kg), K(ATP) blocking with glibenclamide (1.0 mg/kg) injection, ischemia (IS), 30 min ischemia, IPC followed by IS, 8) K(ATP) blocking and IPC followed by IS (KB+IPC+IS), IS and K(ATP) opening (KO+IS). Heart were subjected to ligation of left descending coronary artery and reperfusion in groups of IPC, CP, IS with or without IPC. Immunohistochemistry and Western blotting for PKC-epsilon, NF-kappaB and AP-1 were performed at 3, 6, 24 hours after reperfusion or treatment. Immunoreactivities against PKC-epsilon antibody were observed stronger in the groups of IPC, KO, IPC+IS and KO+IS than groups of KB, IS and KB+IPC+IS. NF-kappaB activation and translocation were only observed in the groups of including 30 min ischemia and reperfusion. AP-1 activation and translocation were opposite to the results of PKC-epsilon activation. In the group of CP, KB, IS and KB+IPC+IS, reactivities of AP-1 antibody were stronger than IPC+IS, KO+IS, and weaker in the groups of S, IPC and KO. These results suggest that K(ATP) opening with IPC or pharmacological methods may direct effect on the PKC-epsilon activation and that K(ATP) blocking has effect on the AP-1 activation and translocation in the heart of ischemiareperfused of rats.
