Determining the molecular weight of two plasmids of S. typhi strains isolated in Vietnam (1995 - 1997 period)
- Author:
Hong Anh Le
- Publication Type:Journal Article
- Keywords:
plasmid
- MeSH:
Plasmids;
Molecular Weight
- From:Journal of Practical Medicine
2002;435(11):52-55
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
This study aimed to extract and type the plasmids of S. typhi strains and antibiogram. 2 S. typhi strains were selected. One multi-antibiotic resistant strain carried only R-plasmid and the other sensitive strain carried 2 smaller plasmids. Both of them were cultured and extracted DNA plasmid. Then 20 different enzymes were analysed to choose proper enzymes. There were 7 enzymes met the requirements. For DNA of larger plasmid, Kpn 1 and Sca 1 cut to 11 fractions. All of them had lower molecular weight than standard DNA. For DNA of smaller plasmid, Kpn 1 enzyme cut to 16 fractions, BamH 1 cut to 10 fractions, Mlu cut to 9 fractions, Cla cut to 11 fractions, and Bgl II cut to 7 fractions. All these fractions had lower weight than standard DNA. Molecular weight of larger plasmid is 188 206bp X 660D = 124 215 960D (124MD). That of smaller plasmid is 99 731bp X 660D = 65 822 460D.
