Emergence and Wide Dissemination of CTX-M-type ESBLs, and CMY-2- and DHA-1-type AmpC beta-Lactamases in Korean Respiratory Isolates of Klebsiella pneumoniae.
10.3346/jkms.2005.20.6.961
- Author:
Jong Hwa YUM
1
;
Shinyoung KIM
;
Hyukmin LEE
;
Dongeun YONG
;
Kyungwon LEE
;
Sang Nae CHO
;
Yunsop CHONG
Author Information
1. Department of Laboratory Medicine, Research Institute of Bacterial Resistance, and Brain Korea 21 Medical Sciences, Yonsei University College of Medicine, Seoul, Korea. leekcp@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
beta-lactamase CTX-M-14;
beta-lactamase CMY-2;
beta-lactamase DHA-1;
AmpC beta-lactamase;
Klebsiella pneumoniae
- MeSH:
Anti-Bacterial Agents/therapeutic use;
Bacterial Proteins/biosynthesis/classification/genetics;
Base Sequence;
Cross Infection/microbiology;
DNA, Bacterial/genetics;
Drug Resistance, Bacterial;
Electrophoresis, Gel, Pulsed-Field;
Genes, Bacterial;
Humans;
Klebsiella Infections/drug therapy/*microbiology;
Klebsiella pneumoniae/classification/*enzymology/genetics/*isolation and purification;
Korea;
Research Support, Non-U.S. Gov't;
Respiratory Tract Infections/drug therapy/*microbiology;
beta-Lactamases/biosynthesis/*classification/genetics
- From:Journal of Korean Medical Science
2005;20(6):961-965
- CountryRepublic of Korea
- Language:English
-
Abstract:
Respiratory isolates of Klebsiella pneumoniae in Korea during 2002-2003 were studied to determine the prevalence and types of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC beta-lactamases (PABLs). ESBL-production was tested by double-disk synergy, and genotypes of beta-lactamases were determined by PCR and sequencing. ESBLs were detected in 28.4% of 373 isolates, and the most prevalent types were SHV-12 (63 isolates) and CTX-M-14 (9 isolates). Forty of 75 ESBL-producers (53.5%) also had PABLs: 21 isolates with CMY-2-like, 17 with DHA-1-like. Pulsed-field gel electrophoresis showed 19 types and 25 of 74 isolates had an identical pattern, indicating nosocomial spread. Dissemination of ESBL- and PABL-producing K. pneumoniae strains in Korea is a particular concern, as it limits the choice of antimicrobial agents for treatment of infections.
