Restoration of Hormone Dependency in Estrogen Receptor - Lipofected MDA-MB-231 Human Breast Cancer Cells.
- Author:
Young Jin SUH
;
Jae Hee CHANG
;
Chung Soo CHUN
- Publication Type:Original Article
- Keywords:
Breast cancer;
Estrogen receptor;
Gene therapy;
Tamoxifen;
Lipofection
- MeSH:
Breast Neoplasms*;
Breast*;
Cell Count;
Cell Line;
DNA, Complementary;
Estradiol;
Estrogen Receptor Modulators;
Estrogens*;
Genetic Therapy;
Humans*;
Phenolsulfonphthalein;
Receptors, Progesterone;
Tamoxifen;
Transfection
- From:Journal of the Korean Cancer Association
1999;31(3):473-482
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The loss of estrogen and progesterone receptors appeats to be associated with a progression to less differentiated and hormone-independent tumors. The gain of hormone independency over time even in estrogen receptor-positive tumors has become another obstacle to endocrine therapy for breast cancer. We tried to regain the hormone dependency in estrogen receptor-negative breast cancer cells by lipofecting estmgen receptor cDNA. MATERIALS AND METHODS: The mutant human estrogen receptor cDNA (pSGS-HEO) was lipofected into estrogen receptor-negative human breast cancer cell line MDA-MB-231, in an attempt to restore their sensitivity to antiestrogen. Then the effects of 17p-estradiol and tamoxifen were studied by counting viable cell numbers after treating the lipofected cell line with either one or together. RESULTS: Culture medium cantaining phenol red, a weak estrogen, has growth advantages compared with culture medium without it. In both culture conditions, cell growth was most profoundly inhibited in 4 days after lipofection with mutant human estrogen receptor cDNA, which was overcome after that day. Tamoxifen, as an antiestrogen, showed a growth inhibitory effect slightly stronger tban combined conditions of tamoxifen and 17- estradiol compared to estrogen-treated group and to control, and the inhibitory effect was lasted 4 days. CONCLUSION: The temporary induction of estrogen receptor by lipofection with pSGS-HEO on estrogen receptor-negative human breast cancer cell line MDA-MB-231 showed negative growth control on these cells by tamoxifen, indicating that liposome-mediated estrogen receptor transfection may be used as a novel therapeutic strategy for hormane independent human breast cancers in the near future.
