Aspergillus oryzae S-03 Produces Gingipain Inhibitors as a Virulence Factor for Porphyromonas gingivalis.
10.4167/jbv.2014.44.2.152
- Author:
Narandalai DANSHIITSOODOL
1
,
2
;
Hideyuki YAMASHITA
;
Masafumi NODA
;
Takanori KUMAGAI
;
Yasuyuki MATOBA
;
Masanori SUGIYAMA
Author Information
1. Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical and Health Sciences, Hiroshima University
2. Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan. sugi@hiroshima-u.ac.jp
- Publication Type:Original Article
- Keywords:
Apergillus oryzae;
Gingipain;
Inhibitor;
Koji
- MeSH:
Aflatoxins;
Anti-Bacterial Agents;
Aspergillus;
Aspergillus oryzae*;
Bacteria;
Connective Tissue;
Genes, Regulator;
Hot Temperature;
Oryza;
Periodontitis;
Porphyromonas;
Porphyromonas gingivalis*;
Soybeans;
Virulence*
- From:Journal of Bacteriology and Virology
2014;44(2):152-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
Oral infection with Porphyromonas (P.) gingivalis causes periodontitis that is manifested by the destruction of gingival connective tissues. Although a few types of antibiotics are effective against the infection, its use induces the appearance of drug-resistant bacteria. The present study shows that the fermented product of Aspergillus (A.) oryzae S-03, cultivated on the fat-removed soybean, inhibits the cell growth of the P. gingivalis. Likewise, the fermented product of the S-03 strain cultured for 26~42 h displays an inhibitory activity to gingipain as a virulence factor of P. gingivalis. The activity is not lost even with heat treatment at 100degrees C for 15 min. We also demonstrate that the S-03 strain exhibits high protease activity. In addition, the strain does not produce aflatoxin because of the loss of a regulatory gene, aflR, necessary for the toxin biosynthesis.
