Genome Size Constraint in Replication and Packaging of Turnip Yellow Mosaic Virus.
10.4167/jbv.2014.44.2.188
- Author:
Hui Bae KIM
1
;
Kwang Hee CHAE
;
Tae Ju CHO
Author Information
1. Department of Biochemistry, Chungbuk National University, Cheongju, Korea. tjcho@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
TYMV;
Plant RNA virus;
Size constraint;
RNA packaging;
Replication
- MeSH:
Blotting, Northern;
Genome;
Genome Size*;
Plant Viruses;
Product Packaging*;
RNA;
RNA, Viral;
Tobacco;
Tymovirus*
- From:Journal of Bacteriology and Virology
2014;44(2):188-196
- CountryRepublic of Korea
- Language:English
-
Abstract:
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. Previously, we have made the recombinant TYMV construct containing a 0.7 kb eGFP gene or a 1.8 kb GUS gene. The genomic RNAs from these constructs were efficiently encapsidated. To examine in more detail whether size constraint exists for replication and packaging of TYMV, we have inserted into the TY-GUS an extra sequence derived from either eGFP or GUS. We also made a recombinant containing RNA1 sequence of Flock house virus. These TYMV recombinants were introduced into Nicotiana benthamiana leaves by agroinfiltration. Northern blot analysis of the viral RNAs in the agroinfiltrated leaves showed that the genomic RNA band from the recombinant TYMV became weaker as longer sequence was inserted. The result also showed that the efficiency of genomic RNA encapsidation decreased sharply when an extra sequence of 2.2 kb or more was inserted. In contrast, the recombinant subgenomic RNA containing an extra sequence of up to 3.2 kb was efficiently encapsidated. Overall, these results show that size constraint exists for replication and encapsidation of TYMV RNA.
