Magnesium vs. machined surfaced titanium - osteoblast and osteoclast differentiation.
10.4047/jap.2014.6.3.157
- Author:
Yong Dae KWON
;
Deok Won LEE
;
Sung Ok HONG
- Publication Type:In Vitro ; Original Article
- Keywords:
Magnesium;
Ion implantation;
Titanium surface;
MC3T3-E1;
Mouse bone marrow-derived macrophages (BMMs)
- MeSH:
Actins;
Alkaline Phosphatase;
Animals;
Bone Remodeling;
Cell Differentiation;
Collagen;
Gene Expression;
Integrin-Binding Sialoprotein;
Macrophages;
Magnesium*;
Mice;
Microscopy, Electron, Scanning;
Osteoblasts*;
Osteocalcin;
Osteoclasts*;
Osteopontin;
Phenotype;
Plasma;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Surface Properties;
Titanium*
- From:The Journal of Advanced Prosthodontics
2014;6(3):157-164
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study focused on in vitro cell differentiation and surface characteristics in a magnesium coated titanium surface implanted on using a plasma ion source. MATERIALS AND METHODS: 40 commercially made pure titanium discs were prepared to produce Ti oxide machined surface (M) and Mg-incorporated Ti oxide machined surface (MM). Surface properties were analyzed using a scanning electron microscopy (SEM). On each surface, alkaline phosphatase (ALP) activity, alizarin red S staining for mineralization of MC3T3-E1 cells, and quantitative analysis of osteoblastic gene expression, were evaluated. Actin ring formation assay and gene expression analysis of TRAP and GAPDH performing RT-PCR were performed to characterize osteoclast differentiation on mouse bone marrow-derived macrophages (BMMs). RESULTS: MM showed similar surface morphology and surface roughness with M, but was slightly smoother after ion implantation at the micron scale. M was more hydrophobic than MM. No significant difference between surfaces on ALP activity at 7 and 14 days were observed. Real-time PCR analyses showed similar levels of mRNA expression of the osteoblast phenotype genes; osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), and collagen 1 (Col 1) in cell grown on MM at 7, 14 and 21 days. Alizarin red S staining at 21 days showed no significant difference. BMMs differentiation increased in M and MM. Actin ring formation assay and gene expression analysis of TRAP showed osteoclast differentiation to be more active on MM. CONCLUSION: Both M and MM have a good effect on osteoblastic cell differentiation, but MM may speed the bone remodeling process by activating on osteoclast differentiation.
