Analysis of Apolipoprotein E Genotypes in Several Demential diseases.
- Author:
Yun Mi PARK
1
;
Young Mi JEON
;
Gye Chul KWON
;
Sun Hoe KOO
;
Jong Woo PARK
Author Information
1. Department of Clinical Pathology, College of Medicine, Chungnam National University, Taejoen, Korea
- Publication Type:Original Article
- Keywords:
Alzheimer's disease;
vascular dementia;
Parkinson's diseases. Apolipoprotein E genotypes
- MeSH:
Alleles;
Alzheimer Disease;
Apolipoprotein E4;
Apolipoproteins E;
Apolipoproteins*;
Dementia;
Dementia, Vascular;
Gene Frequency;
Genotype*;
Humans;
Parkinson Disease;
Polymerase Chain Reaction;
Protein Isoforms;
Risk Factors
- From:Korean Journal of Clinical Pathology
1999;19(6):642-646
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: An increased frequency of the epsilon4 allele in Alzheimer's disease has been reported, which suggested a functional role of apolipoprotein E isoforms in pathophysiology of Alzheimer's disease. The aims of this study were to determine the frequency of ApoE genotypes in various types of dementia such as Alzheimer's disease, vascular dementia, and Parkinson's disease, to relate epsilon4 and assess risk factors for different types of dementia. METHODS: We assessed the frequency of Apolipoprotein E alleles in 29 patients with Alzheimer's disease, 10 patients with vascular dementia and 9 demented patients with Parkinson's disease. The Apolipoprotein E genotype was determined the polymerase chain reaction (PCR) with sequence-specific oligonuculeotide primers (SSOP) and reverse hybridiza- tion using the INNO-LiPA (Line Probe Assay) Apo E typing kit. RESULTS: Allele frequencies of epsilon2, epsilon3, and epsilon4 were : 0.5, 0.72, and 0.23 in Alzheimer's disease; 0, 0.65, and 0.35 in vascular dementia; 0.6, 0.83 and 0.11 in demented patients with Parkinson's disease. Conculsion : These results indicate that the apolipoprotein E4 allele is associated with not only Alzheimer's disease but also vascular dementia, however not associated with Parkinson's disease. No such an association was observed between the apolipoprotein E alleles and age.
