Differential Effect of Vitamin K and Vitamin D Supplementation on Bone Mass in Young Rats Fed Normal or Low Calcium Diet.
10.3349/ymj.2004.45.2.314
- Author:
Jun IWAMOTO
1
;
Tsuyoshi TAKEDA
;
Shoichi ICHIMURA
;
Yoshihiro SATO
;
James K YEH
Author Information
1. Department of Sports Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. jiwamoto@sc.itc.keio.ac.jp
- Publication Type:Original Article
- Keywords:
Calcium;
vitamin K;
vitamin D;
bone growth;
combination therapy
- MeSH:
Age Factors;
Animals;
Antifibrinolytic Agents/*pharmacology;
Bone Density/*drug effects;
Bone Development/*drug effects;
Calcium, Dietary/*pharmacology;
Dietary Supplements;
Female;
Rats;
Rats, Sprague-Dawley;
Vitamin D/*pharmacology;
Vitamin K/*pharmacology
- From:Yonsei Medical Journal
2004;45(2):314-324
- CountryRepublic of Korea
- Language:English
-
Abstract:
The purpose of this study was to clarify the differential effect of vitamin K and vitamin D supplementation on bone mass in young rats fed a normal or low calcium diet. Ninety female Sprague-Dawley rats, 6 weeks of age, were randomized by stratified weight method into nine groups with 10 rats in each group: baseline control, and 0.5% (normal) or 0.1% (low) calcium diet, either alone, or with vitamin K (30 mg/100g, food intake), vitamin D (25microgram/100 g, food intake), or vitamin K + vitamin D. After 10 weeks of feeding, bone histomorphometric analyses were performed on cortical bone of the tibial shaft and cancellous bone of the proximal tibia. Vitamin K supplementation increased the maturation-related cancellous bone gain and retarded the reduction in the maturation-related cortical bone gain in rats fed a low calcium diet, and increased the maturation-related cortical bone gain in rats fed a normal calcium diet. Vitamin D supplementation reduced the maturation-related cancellous bone gain, prevented the reduction in periosteal bone gain, and enhanced the enlargement of the marrow cavity, with no significant effect on the reduction in the maturation-related cortical bone gain in rats fed a low calcium diet, and increased the maturation- related cancellous and cortical bone gains with increased periosteal bone gain in rats fed a normal calcium diet. An additive effect of vitamin K and vitamin D on the maturation- related cortical bone gain was found in rats fed a normal calcium diet. This study shows the differential effects of vitamin K and vitamin D supplementation on cancellous and cortical bone mass in young rats fed a normal or low calcium diet, as well as the additive effect on cortical bone under calcium sufficient condition.
