Decreased Expression of Aquaporin-2 Water Channels in the Kidney in Rats Treated with Chlorpropamide.
- Author:
Soo Wan KIM
1
;
Jong Un LEE
;
Ying Shun LI
;
Yoon Wha OH
;
Nam Ho KIM
;
Ki Chul CHOI
Author Information
1. Department of Physiology, Chonnam National University Medical School, Gwangju, Korea. julee@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Aquaporin water channels;
Chlorpropamide;
Hypoglycemia
- MeSH:
Adenylyl Cyclases;
Animals;
Aquaporin 2*;
Aquaporins*;
Blood Glucose;
Body Weight;
Chlorpropamide*;
Cytoplasm;
Humans;
Hypoglycemia;
Kidney*;
Male;
Membranes;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Nephrology
2002;21(3):375-381
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Whether blood glucose levels may change the regulation of aquaporin(AQP) water channels in the kidney was investigated. METHODS: Male Sprague-Dawley rats were treated with chlorpropamide(40 mg/100 g body weight per day, per oral, for 7 days), and their expression of AQP1-3 and type VI adenylyl cyclase proteins was determined in the kidney. RESULTS: Following the treatment with chlorpropamide, the blood glucose level was significantly decreased compared with that in the control(64+/-8 vs 106+/-7 mg/dL, n=6 each, p < 0.01). Accordingly, the expression of AQP2 proteins was decreased in the cortex, outer medulla, and inner medulla. The AQP2 targeting was not significantly altered, as evidenced by parallel decreases of its expression in the membrane and the cytoplasmic fractions. No significant changes were observed in the expression of either AQP1 or of AQP3. The protein expression of type VI adenylyl cyclase was not significantly altered. CONCLUSION: These results suggest that hypoglycemia attenuates the expression of AQP2 water channels in the kidney.
