Synthetic CDCA Derivatives-Induced Apoptosis of Stomach Cancer Cell Line SNU-1 Cells.
- Author:
Bongkyung MOON
1
;
Min Chan KIM
;
Joo sung PARK
Author Information
1. Department of Family Medicine, Dong-A University College of Medicine, Busan, Korea. jspark@daunet.donga.ac.kr
- Publication Type:Original Article
- Keywords:
Chenodeoxycholic acid;
Stomach neoplasm;
Apoptosis;
Mitochondria;
Caspases
- MeSH:
Apoptosis*;
Blotting, Western;
Caspase 3;
Caspases;
Cell Line*;
Chenodeoxycholic Acid;
Coloring Agents;
Cytochromes c;
DNA;
DNA Fragmentation;
Flow Cytometry;
Humans;
Mitochondria;
Optical Imaging;
Stomach Neoplasms*;
Stomach*
- From:Cancer Research and Treatment
2004;36(2):132-139
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: This study was conducted to explore whether CDCA derivatives induce apoptosis in a stomach cancer cell line, and to dissect the detailed mechanism underlying apoptosis. MATERIALS AND METHODS: The human stomach cancer cell line, SNU-1, cells were treated with the synthetic CDCA derivatives, HS-1199 and HS-1200. DNA and mitochondrial stains were used to detect apoptotic cells by fluorescence imaging or flow cytometry. The caspase-3 activity was measured by Western blotting. RESULTS: Both the HS-1199 and HS-1200 induced decreased viabilities of the SNU-1 cells, in time-dependent manners. The CDCA derivatives demonstrated various apoptosis hallmarks, such as mitochondrial changes reduction of MMP, cytochrome c release, and Smac/ DIABLO translocation), activation of caspase-3 (resulting in the degradation of PARP and DFF45), DNA fragmentation and nuclear condensation. CONCLUSION: The CDCA derivatives, HS-1199 and HS- 1200, both induced apoptosis of the SNU-1 gastric cancer cells in caspase- and mitochondria-dependent fashions. Many important issues relating to their therapeutic applications remain to be elucidated.
