Isolation and Characterization of Chicken NPAS3.
- Author:
Jiheon SHIN
1
;
Hye Yun JEONG
;
Kyung Eun LEE
;
Jaesang KIM
Author Information
- Publication Type:Original Article
- Keywords: NPAS3; CNS; neural tube; chicken; in ovo electroporation
- MeSH: Central Nervous System; Chickens; Electroporation; Embryonic Development; Female; In Situ Hybridization; Motor Neurons; Neural Tube; Neurogenesis; Neurons; Pregnancy; RNA; Stem Cells
- From:Experimental Neurobiology 2010;19(2):71-74
- CountryRepublic of Korea
- Language:English
- Abstract: Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developing neural tube as early as Hamburger-Hamilton (HH) stage 20. Ectopic over-expression of the gene in ovo in the developing chicken neural tube by electroporation had little effect on stem cell population, overall neurogenesis, and motor neuron differentiation. We discuss the implications of our observation.
