Nitric Oxide Is an Essential Mediator for Neuronal Differentiation of Rat Primary Cortical Neuron Cells.
- Author:
Soo Jin OH
1
;
Jee In HEO
;
Yoon Jung KHO
;
Jeong Hyeon KIM
;
Hong Joon KANG
;
Seong Hoon PARK
;
Hyun Seok KIM
;
Jong Yeon SHIN
;
Min Ju KIM
;
Sung Chan KIM
;
Jae Bong PARK
;
Jaebong KIM
;
Jae Yong LEE
Author Information
- Publication Type:Original Article
- Keywords: primary cortical neuron cells; neurite outgrowth; nitric oxide; nitric oxide synthase; butyrate
- MeSH: Animals; Butyrates; Cyclic N-Oxides; Embryonic Structures; Hand; Imidazoles; Neurites; Neurons; Nitric Oxide; Nitric Oxide Synthase; PC12 Cells; Rats; RNA, Messenger
- From:Experimental Neurobiology 2010;19(2):83-89
- CountryRepublic of Korea
- Language:English
- Abstract: Nitric oxide (NO) regulates proliferation, differentiation and survival of neurons. Although NO is reported to involve in NGF-induced differentiation of PC12 cells, the role of NO has not been characterized in primary neuron cells. Therefore, we investigated the role of NO in neuronal differentiation of primary cortical neuron cells. Primary cortical neuron cells were prepared from rat embryos of embryonic day 18 and treated with NMMA (NOS inhibitor) or PTIO (NO scavenger). Neurite outgrowth of neuron cells was counted and the mRNA levels of p21, p27, c-jun and c-myc were measured by RT-PCR. Neurite outgrowth of primary cortical neuron cells was inhibited a little by NOS inhibitor and completely by NO scavenger. The mRNA levels of p21 and p27, differentiation-induced growth arrest genes were increased during differentiation, but they were decreased by NOS inhibitor or NO scavenger. On the other hand, the level of c-jun mRNA was not changed and the level of c-myc mRNA was increased during differentiation differently from previously reported. The levels of these mRNA were reversed in NOS inhibitor- or NO scavenger-treated cells. The level of nNOS protein was not changed but NOS activity was inhibited largely by NOS inhibitor or NO scavenger. These results suggest that NO is an essential mediator for neuronal differentiation of primary cortical neuron cells.
