Studies on Activities of Xenobiotic Metabolic Enzymes in Rats treated with Trichloroethylene.
- Author:
Ki Woong KIM
1
;
Seong Kyu KANG
;
Byung Soon CHOI
;
Jong Seong LEE
;
Jong Sung KIM
;
Young Hahn MOON
Author Information
1. Industrial Health Research Institute Korea Industrial Safety Corporation, Korea.
- Publication Type:Original Article
- Keywords:
Trichloroethylene;
Liver microsomal cytochrome P450;
7-ethoxyresolufin Odeethylase
- MeSH:
Animals;
Chloral Hydrate;
Corn Oil;
Cytochrome P-450 Enzyme System;
Cytochromes;
Electron Transport;
Hand;
Humans;
Injections, Intraperitoneal;
Liver;
Male;
Metabolism;
NADPH-Ferrihemoprotein Reductase;
Oxidoreductases;
Rats*;
Solvents;
Trichloroethylene*
- From:Korean Journal of Occupational and Environmental Medicine
1994;6(2):323-331
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Trichloroethylene (TRI) is one of the most widely used organic solvent in many industries. It is used a degreasing agent, extract and dillution solvents. TRI is metabolized by liver microsomal cytochrome P4SO to reactive intermediates, which are considered responsible for its hepatotoxicity. Cytochrome P450 is a key enzyme in the first step of TRI metabolism resulting in chloral hydrate (CH) formation, which is a rate-limiting step in TRI metabolism. This study was perfomed to find out the change of hepatic cytochrome P450 levels and of electron transport system for P-450 dependent reaction. Intraperitoneal injections of TRI were done to the male Sprague Dawley rats(mean body wights,170+/-10g) in corn oil at the dosage of 150,300,600 mg/kg for 2 days. The results of experiments are following : 1. The contents of microsomal protein, cytochrome P450 and b(5) are tended to be decreased with increasing amount of TRI, but not significantly (p>0.05) . 2. The activity of NADPH-cytochrome P450 reductase is slowly decreased by increasing amount of TRI, but not slgnificantly (p>0.05) . On the other hand, the activity of NADH-b(5) reductase is increased with increasing amount of TRI (p<0.05) 3. The activity of 7-ethoxyresolufin 0-deethylase did not show any critical changes (P>0.05) . This result suggests that the inductive pattern of TRI may not be related to P 450IAl and P450IIB.
