Detection Rate and Prognostic Significance of Human Papillomavirus Type 16 and Type 18 using PCR Method in Uterine Cervical Cancer.
- Author:
Hyo Don SOHN
;
Young Lae CHO
;
Sang Sik CHUN
;
Taek Hoo LEE
;
Bong Jae YOU
;
Han Il JEUNG
;
Moon Kyu KIM
- Publication Type:Original Article
- MeSH:
Carcinoma, Squamous Cell;
Cervix Uteri;
Diagnosis;
DNA;
Female;
Human papillomavirus 16;
Human papillomavirus 18;
Humans*;
Polymerase Chain Reaction*;
Uterine Cervical Neoplasms*
- From:Korean Journal of Gynecologic Oncology and Colposcopy
1994;5(3):19-28
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Human papillomavirus(HPV) has been implicated in the development of uterine cervical cancer. Detectioe of the small amounts of HPV DNA in cervical cells has been very difficult. The polymerase chain reaction(PCR) is a new technique that can specifically amplify target DNA to facilitate its detectiion. PCR technique wes used to detect HPV types 16 and 18 in cervical specimeas obtained from nnormal cervix(20 cases), dysplasia(25 cses), carcinoma in situ(21 cases), microinvasive cancer(ll cases), and invasive cancer(46 cases). And then, case of invasive carcinoma of the uterine cervix were analyzed to determine that the presence of specific human papillomavirus DNA in the neoplasm was a contributing factor to their outcome. The detection rate of HPV 16 DNA in normnal cervix, dysplasia, ClS, microinvasive cancer, and invasive squamous cell carcinoma were 50.0%, 36.0%, 81.0%, 45.5%, and 58.7%, respectively. The detection rate of HPV 18 DNA in normal cervix, dysplasia, CIS, microinvasive cancer, and invasive squamous cell carcinoma were 0.0%, 8,0%, 4.8%, 0.0%, and 19.6%, respectively. of the factors evaluated in invasive cervical cancer, adenocarcinomatous component(p= 0.004) and tumor grade(p=0.015) were found to be correlated with HPV l8 infection. 5 of 8 women whose tumors contained glandular elements had HPV 18 DNA, whereas only 4 of 38 women whose tumors contained only squamous elements showed this infection. 6 of 9 women of HPV l8 infected tumors were grade 3 tumors as compared to only 7 of 28 of HPV 16 infected tumors. Age at diagnosis and nodal status in relation to HPV type 18 exhibited a trend but were not statisitically significant. These observations suggest that HPV type 18 may be associated with a more aggressive form of cervical cancer than HPV type 16.
