The Characteristics of MUC 5 AC in the Corneal Tear Film.
- Author:
Seung Eun JUNG
1
;
So Young KIM
;
Kyoung Yul SEO
;
Jung Won PARK
;
Su Yun JEONG
;
Je Kyung SEONG
;
Eung Kweon KIM
Author Information
1. The Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cornea;
Tear film;
Mucin;
MUC5AC
- MeSH:
Cornea;
Goblet Cells;
Humans;
Immunohistochemistry;
Mucins;
Tears*
- From:Journal of the Korean Ophthalmological Society
2000;41(6):1304-1309
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The mucous layer located on the cornea epithelial surface is mainly composed of mucin.It is unique in being composed of a central protein back bone surrounded by an outermost dense oligosaccharide matrix, yet its pre-cise composition is still unknown. Conjunctival goblet cells have been known to express the mucin MUC5AC. We attempted to reveal the presence of the secretory mucin MUC5AC as a component of the mucous layer in the rabbit corneal tear film. An antibody to a MUC5AC peptide was used to detect the MUC5AC mucin present in the tear film by immunohistochemistry. Human conjuncti-val impression cytology and immunohistochemistry were done with the same antibody. The MUC5AC mucin was observed in the precorneal tear film and con-junctival goblet cells by immunohistochemistry.The MUC5AC mucin is a component of the mucous layer and is produced by the goblet cells of con-junctiva.
