Evaluation of Two Beta2-microglobulin Reagents by Turbidimetric Immunoassay.
10.3343/kjlm.2007.27.6.414
- Author:
Kyung sun PARK
1
;
So Young KANG
;
Woo In LEE
Author Information
1. Department of Laboratory Medicine, Kyung Hee University College of Medicine, Seoul, Korea. syandmy@hotmail.com
- Publication Type:Original Article ; English Abstract ; Evaluation Studies
- Keywords:
Beta2-microglobulin;
Turbidimetric immunoassay;
Chemiluminescent immunoassay
- MeSH:
Aged;
Chemiluminescent Measurements;
Enzyme Multiplied Immunoassay Technique;
Female;
Humans;
Immunoassay/*methods;
Male;
Middle Aged;
Nephelometry and Turbidimetry;
Reagent Kits, Diagnostic;
Regression Analysis;
Sensitivity and Specificity;
beta 2-Microglobulin/*analysis
- From:The Korean Journal of Laboratory Medicine
2007;27(6):414-419
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: An increased level of beta2-microglobulin (beta2M) is seen in diseases such as lymphoproliferative diseases, renal diseases, solid tumors, liver diseases, certain viral infection, or chronic inflammatory diseases, etc. In this study, we evaluated a quantitative beta2M assay for precision and linearity using an automated turbidimetric immunoassay (TIA) by Hitachi 7600-110 (Hitachi High Technologies Co., Japan). The TIA of beta2M was compared with a chemiluminescent immunoassay (CLIA) by Immulite 2000 (Diagnostic Products Corporation, USA). METHODS: Two TIAs, the Hitachi 7600-110 with Roche reagent (Roche-TIA) and the Hitachi 7600- 110 with HBI reagent (HBI-TIA), were evaluated for within-run precision, within-day precision, betweendays precision, and linearity. With 68 serum samples, two TIAs were compared with Immulite 2000 using DPC reagent (DPC-CLIA). These data were analyzed by Passing-Bablok analysis and Bland- Altman analysis. RESULTS: The coefficients of variation (CVs) of within-run precision, within-day precision, and between- days precision were less than 7% in all groups. The linearity tests of the two TIAs were maintained well (Roche-TIA: R2=0.9952; HBI-TIA: R2=0.9946). The comparison study indicated good correlations (Roche-TIA/DPC-CLIA: r=0.9738, y=0.9625x-0.0375; HBI-TIA/DPC-CLIA: r=0.9725, y= 1.1000x-0.3100). In Bland-Altman analysis, less than 2SD differences were observed between the two groups. CONCLUSIONS: Both Roche-TIA and HBI-TIA showed a good precision and excellent correlations with DPC-CLIA; therefore, TIA could be used in the routine laboratory to determine a quantitative analysis of beta2M.
