Coproduction of qnrB and armA from Extended-Spectrum beta-lactamase-producing Klebsiella pneumoniae.
10.3343/kjlm.2007.27.6.428
- Author:
Moon Hee KIM
1
;
Ji Youn SUNG
;
Jong Woo PARK
;
Gye Cheol KWON
;
Sun Hoe KOO
Author Information
1. Department of Laboratory Medicine, Sun General Hospital, Daejeon, Korea.
- Publication Type:Original Article ; English Abstract
- Keywords:
Extended-spectrum beta-lactamase;
qnrB;
armA;
Klebsiella pneumoniae
- MeSH:
Bacterial Proteins/biosynthesis/*genetics;
Disk Diffusion Antimicrobial Tests;
Drug Resistance, Bacterial/genetics;
Humans;
Klebsiella Infections/microbiology;
Klebsiella pneumoniae/drug effects/*genetics/isolation & purification;
Methyltransferases/biosynthesis/*genetics;
beta-Lactamases/biosynthesis/drug effects/*genetics
- From:The Korean Journal of Laboratory Medicine
2007;27(6):428-436
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Multidrug-resistant Enterobacteriaceae is a worldwide problem. Although various resistance mechanisms have been recognized with increasing frequency, only a few cases of triple resistance of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been reported. This study was designed to evaluate the coexistence of qnr (qnrA, qnrB, and qnrS) and 16S rRNA methylase (armA, rmtA, rmtB, and rmtC) in ESBL-producing K. pneumoniae. METHODS: We tested 44 isolates of ESBL-producing K. pneumoniae at Chungnam National University Hospital from March to September 2006. Antimicrobial susceptibilities were tested by broth microdilution method, and transconjugation test was performed using E. coli J53 with azide resistance. Search for qnr (qnrA, qnrB, and qnrS) and 16S rRNA methylase (armA, rmtA, rmtB, and rmtC) genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. Epidemiologic study was performed by Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). RESULTS: All ESBL-positive strains produced qnrB; however, armA was detected in 68.2%. The coproduction rate of qnrB and armA in ESBL-producing K. pneumoniae was 68.2%. Two types (A and B) were dominant in ERIC-PCR results. CONCLUSIONS: K. pneumoniae producing qnrB, armA, and ESBL are spreading widely.
