Development of a Web-based Program for the Identification of Human Leukocyte Antigen Antibody Specificities.
10.3343/kjlm.2007.27.6.458
- Author:
Choong Hwan CHA
1
;
Heung Bum OH
;
Myeong Hee KIM
;
Jeong Min CHAE
;
Soon Young JUNG
Author Information
1. Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea. hboh@amc.seoul.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Panel reactive antibody (PRA);
Tail analysis;
Human leukocyte antigens (HLA);
Antibody specificity
- MeSH:
Algorithms;
*Antibody Specificity/genetics;
HLA Antigens/genetics/*immunology;
Histocompatibility Testing;
Humans;
Internet;
*Software
- From:The Korean Journal of Laboratory Medicine
2007;27(6):458-463
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.
