Immunohistochemical Study of the Endotoxin Induced Uveitis in Rat.
- Author:
Jong Moon PARK
1
;
Ji Myong YOO
;
Joon Kyeong SONG
Author Information
1. Department of Ophthalmology, College of Medicine, Gyeong Sang National University, Korea.
- Publication Type:Original Article
- Keywords:
Endotoxin;
Immunohistochemical technique;
T and B lymphocyte
- MeSH:
Animals;
Antibodies;
Ciliary Body;
Frozen Sections;
Immunoglobulin G;
Immunoglobulin M;
Lymphocytes;
Rats*;
T-Lymphocytes;
Uveitis*
- From:Journal of the Korean Ophthalmological Society
1992;33(6):583-588
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Using immunohistochemical technique, we observed time-sequentially to T and B lymphocyte in the ciliary body of the endotoxin induced uveitis in rat. The primary antibodies for immunohistochemical stain of the lymphocyte were IgM heavy chain, IgG heavy chain, T suppressor/cytotoxic (CD8) and T helper/macrophage (CD4) was performed in the frozen section. The intensity of immunohistochemical reaction for surface IgM positive cells increased from 12hours and reached most strong intensities at 24hours after endotoxin injection and then decreased rapidly, but the intensity for surface IgG positive cells increased from 48hours and sustained 96hours. There were no significant changes of intensity of immunohistochemical reaction for T cells except slightly increased T suppressor/cytotoxic cells (CD8).
