Effects of 5-hydroxytryptamine on Rocuronium-induced Neuromuscular Blockade in a Rat Phrenic Nerve-hemidiaphragm Preparation.
10.4097/kjae.2007.52.4.438
- Author:
Woo Jong CHOI
1
;
Hye Jin KIM
;
Yoon Kyung LEE
;
Hong Seuk YANG
Author Information
1. Department of Anesthesiology and Pain Medicne, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea. hsyang@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
cross reaction;
neuromuscular blockade;
rocuronium;
5-hydroxytryptamine
- MeSH:
Animals;
Baths;
Cross Reactions;
Humans;
Ligand-Gated Ion Channels;
Male;
Neuromuscular Blockade*;
Peritoneal Cavity;
Phrenic Nerve;
Rats*;
Rats, Sprague-Dawley;
Receptors, Nicotinic;
Serotonin*;
Transducers
- From:Korean Journal of Anesthesiology
2007;52(4):438-442
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The 5-hydroxytryptamine 3 receptor (5-HT3R) is a member of a superfamily of ligand-gated ion channels which has structural similarities and common evolutionary origin to those of the nicotinic acetylcholine receptor (nAChR). 5-hydroxytryptamine (5-HT) and muscle relaxants may have cross reaction. Rocuronium is a non-depolarizing neuromuscular blocking agent which has a rapid onset. The aim of this study was to examined the effects of 5-HT on rocuronium-induced neuromuscular blockade in a rat phrenic nerve-hemidiaphragm preparation. METHODS: Institutional approval was obtained for the experimental procedure. Fifty male Sprague-Dawley rats (150-200 g) were divided into 5 groups; the control, and 0.1, 1, 10, and 20microgram/ml of 5-HT. The animals were injected with phentobarbital at 40 mg/kg into the peritoneal cavity. The hemidiaphragm with the phrenic nerve was dissected and then mounted in a bath containing 100 ml Krebs solution at room temperature. The phrenic nerve was stimulated at the supramaximal intensity using a Grass(R) S88 stimulator via an SIU5 isolation unit. The twitch height was measured and recorded using a precalibrated Grass(R) FT88 force displacement transducer and recorded with a Grass(R) 79 polygraph, respectively. In the cumulative dose-response study, the rocuronium 100microgram/dl and each dose of 5-HT were administered simultaneously administered, with additional 50microgram/dl incremental doses of rocuronium were added to obtain grteater than 95% neuromuscular twitch inhibition. The ED5, ED50, ED90, and ED95 of rocuronium in each group were calculated using a probit model. RESULTS: The ED50, ED90, and ED95 of rocuronium were significantly reduced in 5-HT 20microgram/ml group (P < 0.05), but no differences were observed with the other 5-HT groups compared to the control groups. CONCLUSIONS: 5-HT at 20microgram/ml enhanced the neuromuscular blockade of rocuronium.
