Influence of Activin and Follistatin on Expression of Insulin-like Growth Factor (IGF)-I, II, Insulin-like Growth Factor Binding Protein (IGFBP)-1, 2, and 3 mRNA in Cultured Mouse Granulosa Cells.
- Author:
Hee Dong CHAE
1
;
Seok Ho HONG
;
Yun Hyun CHO
;
Young Mi OH
;
Bang Hyun LEE
;
Sung Hoon KIM
;
Chung Hoon KIM
;
Byung Moon KANG
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Activin;
Follistatin;
Insulin-like growth factor system;
Cultured mouse granulosa cells
- MeSH:
Activins*;
Animals;
Carrier Proteins*;
Female;
Follistatin*;
Granulosa Cells*;
Insulin-Like Growth Factor Binding Protein 1;
Insulin-Like Growth Factor Binding Protein 2;
Insulin-Like Growth Factor Binding Protein 3;
Insulin-Like Growth Factor Binding Proteins;
Insulin-Like Growth Factor I;
Mice*;
Physiology;
RNA, Messenger*
- From:Korean Journal of Obstetrics and Gynecology
2003;46(3):592-599
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To investigate the influence of activin and follistatin on the expression of IGF (insulin-like growth factor)-I, II, IGFBP (insulin-like growth factor binding protein)-1, 2, and 3 mRNA in cultured mouse granulosa cells MATERIALS AND METHODS: The granulosa cells were obtained from the mouse and cultured for 6 days with 10 ng/ml of activin, 10 ng/ml of follistatin, and 10 ng/ml of activin with 10 ng/m of follistatin, respectively. The cells not treated with activin or follistatin served as control. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to examine the expression of IGF-I, II, IGFBP-1, 2, and 3 mRNA. Results were analyzed with Kolmogorov-Smirnov test and analysis of variance (ANOVA) and statistical significance was defined as p<0.05. RESULTS: The expression of IGF-I and II mRNA were not different significantly. However, the expression of IGFBP-3 mRNA was significantly increased in the follistatin group compared to the control group (p<0.05) and significantly decreased in the activin with follistatin group compared to the control group (p<0.05). The expression of IGFBP-1 mRNA was seemed to be increased in the activin group and decreased in the follistatin group compared to the control group, respectively (p=0.07, p=0.07). The expression of IGFBP-2 and 3 mRNA were seemed to be decreased in the activin group compared to the control group (p=0.06, p=0.07, respectively). CONCLUSION: Activin and follistatin might play a role as regulators of mouse ovarian physiology by modulating the IGF system, especially IGFBPs.
