Effects of Recombinant Human Epidermal Growth Factor (rhEGF) on Experimental Radiation-Induced Oral Mucositis in Rats.
- Author:
Kwonil JUNG
1
;
Sunhee KIM
;
Soo Young MOON
;
Yeon Wha KIM
;
Joon Pio HONG
;
Hyun Sook KIM
;
Sang wook LEE
Author Information
1. Department of Toxicology, Daewoong Pharmaceutical Co., LTD., Yongin, Korea.
- Publication Type:Original Article
- Keywords:
Oral mucositis;
Radiation;
Epidermal growth factor;
Rat model
- MeSH:
Animals;
Drug Therapy;
Eating;
Epidermal Growth Factor*;
Hand;
Head;
Head and Neck Neoplasms;
Humans*;
Models, Animal;
Mouth Mucosa;
Rats*;
Stomatitis*;
Survival Rate;
Ulcer;
Weight Loss
- From:The Journal of the Korean Society for Therapeutic Radiology and Oncology
2006;24(1):67-76
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Oral mucositis is a common toxicity of radiation or chemotherapy, which is used a treatment for head and neck cancer. We investigated effects of recombinant human epidermal growth factor (rhEGF) on radiation-induced oral mucositis in rat model. MATERIALS AND METHODS: Spraque-Dawley rats (7 per group) exposed to a single dose of 25 Gy (day 0) on their head, except for one group, were randomly divided into un-treated, vehicle-treated, and two rhEGF- treated groups. Rats were topically applied with rhEGF (15 or 30 microgram/oral cavity/day) or vehicle to their oral mucosa. Survival rate of rats, weight changes, and food intakes were examined from day 0 to 18 after radiation. Histology study was performed from oral mucosa of rats at day 7 and 18 after radiation. RESULTS: rhEGF-treated groups (15 or 30 microgram/day) showed all survival rate 33%, whereas un-treated and vehicle-treated groups showed all survival rate 0% at the end of experiment. rhEGF-treated groups statistically had less weight loss compared to vehicle-treated group from day 2 to 7 after radiation. Food intake of rats with rhEGF treatment turned to increase at day 14 after radiation. At 7 day after radiation, un-treated and vehicle-treated groups showed severe pseudomembraneous or ulcerative oral mucositis. On the other hand, rhEGF-treated groups had no more than cellular swelling and degeneration of epidermal cells in oral mucosa of rats. CONCLUSION: These results suggest that rhEGF has significantly positive effects on radiation-induced oral mucositis in rats. rhEGF display a therapeutic potential on a clinical level.
