The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines.
10.3346/jkms.1993.8.2.153
- Author:
Chang Dae BAE
1
;
Seong Eun PARK
;
Yeon Sun SEONG
;
Seung Won KIM
;
Joo Bae PARK
;
Jae Gab PARK
Author Information
1. Department of Biochemistry, Seoul National University College of Medicine, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
c-erbB-2 oncogene-stomach cancer-post-transcriptional regulation
- MeSH:
Amino Acid Sequence;
Humans;
Molecular Sequence Data;
Protein-Tyrosine Kinases/*biosynthesis;
Proto-Oncogene Proteins/*biosynthesis/genetics/immunology;
RNA, Messenger/analysis;
Receptor, Epidermal Growth Factor/*biosynthesis/genetics/immunology;
Receptor, erbB-2;
Receptors, Cell Surface/*biosynthesis;
Stomach Neoplasms/genetics/*metabolism;
Tumor Cells, Cultured
- From:Journal of Korean Medical Science
1993;8(2):153-159
- CountryRepublic of Korea
- Language:English
-
Abstract:
c-erbB-2 oncogene encodes a growth factor receptor whose amino acid sequence has extensive homology with human epidermal growth factor receptor. It is frequently overexpressed in human breast, ovary, lung, and stomach cancers, where its overexpression is related significantly to the prognosis. Tl investigate the possible role of c-erbB-2 oncogene in the oncogenesis of stomach cancer, we examined the genetic alterations of c-erbB-2 oncogene in 4 stomach cancer cell lines, SNU-1, SNU-5, SNU-16 and KATO III. There were no differences in c-erbB-2 mRNA level as well as c-erbB-2 gene copy number among them. But gp185-erbB-2, c-erbB-2 gene product, was increased from 2- to 4-fold in SNU-1 and SNU-5 cells, compared with that in SNU-16 or KATO III cells. Our results suggest that post-transcriptional regulation of gp185erbB-2 expression may underlie gp185erbB-2 overexpression in cancer cells.
