The Effects of Calcium Phosphate Glass on Mouse Calvarial Cell.
10.5051/jkape.2004.34.1.49
- Author:
Min Kyoung KIM
1
;
Chang Sung KIM
;
Doug Youn LEE
;
Yong Keun LEE
;
Kyoo Sung CHO
;
Jung Kiu CHAI
;
Chong Kwan KIM
;
Seong Ho CHOI
Author Information
1. Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
calcium phosphate glass;
mouse calvarial cell;
MTT assay;
collagen assay;
ALP activity
- MeSH:
Animals;
Bone Morphogenetic Proteins;
Calcium*;
Collagen;
Glass*;
Guided Tissue Regeneration;
Intercellular Signaling Peptides and Proteins;
Mice*;
Periodontium;
Regeneration;
Transplants
- From:The Journal of the Korean Academy of Periodontology
2004;34(1):49-59
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The goal of periodontal treatment is not only to arrest the progression of the disease but also to promote the functional, esthetic regeneration of the periodontium. Flap operation, bone graft, guided tissue regeneration, growth factors and bone morphogenetic protein have been used for this purpose. Among these techniques of regeneration, alloplastic graft, especially calcium phosphate is getting more attention recently. The purpose of this study was to evaluate the effects of calcium phosphate glass on mouse calvarial cell in vitro. The toxicity of calcium phosphate glass was measured using MTT assay, the synthesis of collagen was measured using collagen assay, and ALP activity was measured. The experimental groups were cultured with calcium phosphate glass(both AQ-, and HT-CPG) in concentration of 0.01, 0.02, 0.1, 0.2g/ml. The results are as follows 1. In concentrations not exceeding 0.02g/ml, both the groups(AQ-CPG, HT-CPG) didn't show any toxicity on mouse calvarial cell(p<0.05). 2. In both the experimental groups are the concentration of 0.02g/ml, collagen expressions were significantly up-regulated (p<0.05). 3. In both the experimental groups are the concentration of 0.02g/ml, ALP activity was not significantly upregulated, but ALP activity in both experimental groups were greater than control group(p<0.05). The results suggested that the use of calcium phosphate glass may promotes periodontal regeneration. Ongoing studies are necessary in order to determine their regeneration effects.
