Anticancer effect of mastic on human oral cancer cells.
10.11149/jkaoh.2016.40.3.143
- Author:
Ji Hye KIM
1
;
Jung Hyuck CHOI
;
Youn Sook JUNG
;
Min Jeong CHO
;
Young Eun LEE
;
Dong Ok PARK
;
Keun Bae SONG
Author Information
1. Department of Preventive Dentistry, Kyungpook National University School of Dentistry, Daegu, Korea. kbsong@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Mastic;
Oral cancer
- MeSH:
Apoptosis;
Cell Count;
Greece;
Humans*;
Mouth Neoplasms*;
Pistacia
- From:Journal of Korean Academy of Oral Health
2016;40(3):143-148
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Mastic is a resinous extract from the stem and main leaves of Pistacia lentiscus, grown only in the Chios island of Greece. Mastic has antibacterial, anti-inflammatory, anticancer, and anti-ulcer activities. Although mastic has been widely studied, its inhibitory effect against cancer cells, especially oral cancer cells, has not been elucidated. The purpose of this study was to assess the anticancer effects of mastic on human oral cancer YD-10B cells. METHODS: YD-10B cells were cultured in 0, 1, 2, 5, and 10 µg/mL mastic for 24 h. Cell count, viability, morphology, colony-forming assay, and DAPI staining were analyzed. RESULTS: Mastic treatment of YD-10B cells resulted in a dose-dependent inhibition of cell growth, and almost all the cells in the 10 µg/mL culture were dead (P<0.05). Mastic treatment induced a morphological change and nuclear fragmentation in the YD-10B cells, and inhibited colony formation of YD-10B cells in a dose-dependent manner. CONCLUSIONS: These results indicate that mastic exhibited anticancer effects on the YD-10B cells through changes in cell morphology and apoptosis.
