The Regulation of MMP-2 and -14 Expressions by TGF-beta in Lens Epithelial Cells.
- Author:
Min Jung SON
;
Jong Tak KIM
;
Choun Ki JOO
- Publication Type:Original Article
- Keywords:
Lens epithelial cell;
Matrix metalloproteinase;
Nuclear and anterior polar cataract;
Transforming growth factor-beta
- MeSH:
Animals;
Capsules;
Cataract;
Cell Movement;
Epithelial Cells*;
Epithelial-Mesenchymal Transition;
Fluorescent Antibody Technique;
Humans;
Matrix Metalloproteinases;
Rats;
src-Family Kinases;
Transforming Growth Factor beta*
- From:Journal of the Korean Ophthalmological Society
2006;47(7):1110-1116
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: TGF-beta is a key regulator of epithelial-mesenchymal transition. Among the TGF-beta responses, cell migration is closely associated with the expression of matrix metalloproteinases (MMPs). Therefore, we determined which MMPs are regulated by TGF-beta and examined the TGF-beta signaling involved in this event, focusing on Src family tyrosine kinases (SFKs) METHODS: First we examined the expression of MMPs in rat lens explant culture treated with TGF-beta and LECs attached to the anterior capsules of patients with nuclear (N), anterior polar (AP) cataracts using RT-PCR and immunofluorescence staining. It was examined whether the expression of MMPs is regulated by SFKs. RESULTS: The study using RT-PCR and immunofluorescence staining showed the expression of MMP-2 and -14 in explants and the expression of MMP-14 LECs of AP cataracts. The expression of MMP-2 and -14 was blocked by PP2 in explants. Furthermore, the activated form of SFKs was observed in LECs of AP cataracts by immunofluorescence staining. CONCLUSIONS: We suggest a novel role of SFKs signaling in the expression of MMP-14 induced by TGF-beta.
