Co-expression of Survivin and Bcl-2 in Primary Brain Tumors: Their Potential Effect on Anti-apoptosis.
- Author:
Je Il RYU
1
;
Choong Hyun KIM
;
Jin Hwan CHEONG
;
Koang Hum BAK
;
Jae Min KIM
;
Suck Jun OH
Author Information
1. Department of Neurosurgery, School of Medicine, Hanyang University, Seoul, Korea. kch5142@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Survivin;
Bcl-2;
Apoptosis;
Brain neoplasm;
Co-expression
- MeSH:
Apoptosis;
Blotting, Western;
Brain Neoplasms*;
DNA Fragmentation;
Humans;
Meningeal Neoplasms
- From:Journal of Korean Neurosurgical Society
2006;40(1):1-5
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Survivin is an inhibitor of apoptosis protein(IAP), which inhibits apoptosis through a pathway distinct from the Bcl-2 family members. Overexpression of survivin and Bcl-2 have been commonly reported in human neoplasms. The authors investigate whether there is a synergistic effect on the anti-apoptosis rate of primary brain tumors "in situ" based on the co-expression of survivin and Bcl-2. METHODS: One hundred and two brain tumor patients who had been resected were included in this study. Survivin and Bcl-2 were detected by Western blotting analysis, while apoptosis was examined by DNA fragmentation analysis. An anti-apoptotic rate was assessed in these brain tumor samples based on the expression of survivin and Bcl-2 or co-expression of both. RESULTS: Survivin and Bcl-2 were expressed in 57(55.9%) and 53(52.0%) of 102 brain tumor samples studied respectively, and co-expressed in 31(30.4%). The percentage of astrocytic and meningeal tumors expressing survivin was significantly correlated with histological grades; however, Bcl-2 was not correlated (p=0.106). The anti-apoptotic rate in primary brain tumors with survivin, Bcl-2, and both was detected in 49(86.0%) of 57 samples, 42(79.9%) of 53 samples, and 27(87.1%) of 31 samples, respectively. Their difference in the frequency of anti-apoptosis was not significant. CONCLUSION: Survivin or Bcl-2 is involved in the anti-apoptosis. However, it suggests that co-expression of survivin and Bcl-2, together, have no synergistic effect on the anti-apoptotic properties of the primary brain tumors.
