An Association between 609 C --> T Polymorphism in NAD(P)H: Quinone Oxidoreductase 1 (NQO1) Gene and Blood Glucose Levels in Korean Population.
- Author:
Dohee KIM
1
Author Information
- Publication Type:Original Article
- Keywords: Blood glucose; Human NQO1; Single nucleotide polymorphism
- MeSH: Benzoquinones; Blood Glucose; Blood Pressure; Body Mass Index; Electrons; Fasting; Genotype; Glucose; Homeostasis; Humans; Insulin; Oxidative Stress; Oxidoreductases; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Tissue Donors
- From:Korean Diabetes Journal 2009;33(1):24-30
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: NAD(P)H: quinone oxidoreductase 1 (NQO1), which is an obligate two-electron reductase that utilizes NAD(P)H as an electron donor and is involved in the protection against oxidative stress, is likely involved in beta-cell destruction. We evaluated the frequency of the NQO1 polymorphism and its association with blood glucose levels. METHODS: Genotypes were determined using a polymerase chain reaction restriction fragment length polymorphism-based assay in 56 patients and 48 healthy subjects. Fasting blood glucose, insulin, and lipid profiles were measured and homeostasis model assessment (HOMA)-insulin resistance (IR) was calculated from fasting glucose and insulin levels in the healthy subjects. RESULTS: The genotype frequencies of NQO1 polymorphism were C/C (56.7%), C/T (42.3%), and T/T (1.0%). There were no associations between the NQO1 polymorphism and body mass index, blood pressure, lipid profile, HbA1c, postprandial glucose, and HOMA-IR. However, NQO1 mutants (C/T and T/T) showed weak but significantly higher fasting blood glucose levels compared with wild type (C/C). CONCLUSION: Our data suggest that NQO1 609 C --> T polymorphism may be associated with glucose metabolism.
