Preimplantation Development and Apoptosis of Mouse Embryos in the Medium Containing Extracellualr Matrix.
- Author:
Byung Moon KANG
;
In Pyo SOHN
;
Byung Mok CHUNG
;
Kyoo Wan CHOI
;
Myung Chan GYE
- Publication Type:Original Article
- MeSH:
Animals;
Apoptosis*;
Blastocyst;
Cell Count;
Eggs;
Embryonic Structures*;
In Situ Nick-End Labeling;
Mice*;
Ovum;
Sarcoma;
Zygote
- From:Korean Journal of Fertility and Sterility
2000;27(3):253-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm(EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. METHOD: Late Pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assessed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. RESULTS: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. CONCLUSION: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.
