In Vivo Effects of Adipose-Derived Stem Cells in Inducing Neuronal Regeneration in Sprague-Dawley Rats Undergoing Nerve Defect Bridged with Polycaprolactone Nanotubes.
10.3346/jkms.2014.29.S3.S183
- Author:
Dong Yeon KIM
1
;
Yong Seong CHOI
;
Sung Eun KIM
;
Jung Ho LEE
;
Sue Min KIM
;
Young Jin KIM
;
Jong Won RHIE
;
Young Joon JUN
Author Information
1. Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea. joony@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Stem Cells;
Adipose Tissue;
Nanotubes;
Matrix Attachment Regions;
Peripheral Nerves;
Regeneration;
Polycaprolactone
- MeSH:
Adipose Tissue/cytology;
Animals;
Cell Differentiation;
Electromyography;
Male;
Nanotubes;
*Nerve Regeneration;
Nerve Tissue Proteins/immunology;
Nestin/immunology;
Neural Conduction/physiology;
Peripheral Nerve Injuries/*surgery;
Phosphoprotein Phosphatases/immunology;
Polyesters/*therapeutic use;
Rats;
Rats, Sprague-Dawley;
Sciatic Nerve/injuries/surgery;
Stem Cell Transplantation/*methods;
Stem Cells/*cytology;
Tissue Engineering/methods
- From:Journal of Korean Medical Science
2014;29(Suppl 3):S183-S192
- CountryRepublic of Korea
- Language:English
-
Abstract:
There have been many attempts for regeneration of peripheral nerve injury. In this study, we examined the in vivo effects of non-differentiated and neuronal differentiated adipose-derived stem cells (ADSCs) in inducing the neuronal regeneration in the Sprague-Dawley (SD) rats undergoing nerve defect bridged with the PCL nanotubes. Then, we performed immunohistochemical and histopathologic examinations, as well as the electromyography, in three groups: the control group (14 sciatic nerves transplanted with the PCL nanotube scaffold), the experimental group I (14 sciatic nerves with the non-differentiated ADSCs at a density of 7x105 cells/0.1 mL) and the experimental group II (14 sciatic nerves with the neuronal differentiated ADSCs at 7x105 cells/0.1 mL). Six weeks postoperatively, the degree of the neuronal induction and that of immunoreactivity to nestin, MAP-2 and GFAP was significantly higher in the experimental group I and II as compared with the control group. In addition, the nerve conduction velocity (NCV) was significantly higher in the experimental group I and II as compared with the control group (P=0.021 and P=0.020, respectively). On the other hand, there was no significant difference in the NCV between the two experimental groups (P>0.05). Thus, our results will contribute to treating patients with peripheral nerve defects using PCL nanotubes with ADSCs.
