Neurotoxicity Screening in a Multipotent Neural Stem Cell Line Established from the Mouse Brain.
10.3346/jkms.2010.25.3.440
- Author:
Yong Soo CHOI
1
;
Min Cheol LEE
;
Hyung Seok KIM
;
Kyung Hwa LEE
;
Yeoung Geol PARK
;
Hyun Kyung KIM
;
Han Seong JEONG
;
Myeong Kyu KIM
;
Young Jong WOO
;
Seung Up KIM
;
Jae Kyu RYU
;
Hyun Beom CHOI
Author Information
1. Department of Pathology, Chonnam National University Medical School, Gwangju, Korea. mclee@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Growth Factors;
Brain;
Nestin;
Stem Cells;
Culture
- MeSH:
Animals;
Brain/*cytology;
Buthionine Sulfoximine/pharmacology;
Cell Differentiation;
Cell Line;
Cell Lineage;
Cytokines/pharmacology;
Enzyme Inhibitors/pharmacology;
Excitatory Amino Acid Agonists/pharmacology;
Glutamic Acid/pharmacology;
Humans;
Hydrogen Peroxide/pharmacology;
Intercellular Signaling Peptides and Proteins/pharmacology;
Kainic Acid/pharmacology;
Mice;
Mice, Inbred BALB C;
Multipotent Stem Cells/cytology/*drug effects/physiology;
Neuroglia/cytology/drug effects/physiology;
Neurons/cytology/*drug effects/physiology;
Neurotoxins/*pharmacology;
Oxidants/pharmacology;
Phenotype;
Reactive Oxygen Species/metabolism
- From:Journal of Korean Medical Science
2010;25(3):440-448
- CountryRepublic of Korea
- Language:English
-
Abstract:
Neural stem cells (NSCs) have mainly been applied to neurodegeneration in some medically intractable neurologic diseases. In this study, we established a novel NSC line and investigated the cytotoxic responses of NSCs to exogenous neurotoxicants, glutamates and reactive oxygen species (ROS). A multipotent NSC line, B2A1 cells, was established from long-term primary cultures of oligodendrocyte-enriched cells from an adult BALB/c mouse brain. B2A1 cells could be differentiated into neuronal, astrocytic and oligodendroglial lineages. The cells also expressed genotypic mRNA messages for both neural progenitor cells and differentiated neuronoglial cells. B2A1 cells treated with hydrogen peroxide and L-buthionine-(S,R)-sulfoximine underwent 30-40% cell death, while B2A1 cells treated with glutamate and kainate showed 25-35% cell death. Cytopathologic changes consisting of swollen cell bodies, loss of cytoplasmic processes, and nuclear chromatin disintegration, developed after exposure to both ROS and excitotoxic chemicals. These results suggest that B2A1 cells may be useful in the study of NSC biology and may constitute an effective neurotoxicity screening system for ROS and excitotoxic chemicals.
