An Analysis for Angiotensin Converting Enzyme Gene Polymorphism in Formalin-fixed, Paraffin-embedded Tissues from Patients with Sarcoidosis.
- Author:
Tae Sook KIM
1
;
Hee Yeon HONG
;
In chul LEE
Author Information
1. Department of Pathology, Inha University College of Medicine, Incheon, Korea. tskim@inha.ac.kr
- Publication Type:Original Article
- Keywords:
Sarcoidosis;
Gene;
Polymorphism;
Angiotensin converting enzyme;
Polymerase chain reaction
- MeSH:
Angiotensins*;
Ethnic Groups;
Gene Frequency;
Genotype;
Granuloma;
Homozygote;
Humans;
Lung;
Lymph Nodes;
Peptidyl-Dipeptidase A*;
Polymerase Chain Reaction;
Sarcoidosis*
- From:Korean Journal of Pathology
2003;37(4):225-231
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Sarcoidosis is a systemic disease characterized by nonnecrotizing granulomas involving the lung and hilar lymph nodes. Serum angiotensin converting enzyme (sACE) levelsin patients with sarcoidosis have been implicated as an indicator of granuloma burden.Recently, it has been found that ACE gene insertion/deletion (ID) polymorphism affects sACE levels in healthy individuals. Moreover, reported sACE levels were highest in the deletion/deletion(DD) genotype. Previous studies to investigate the distribution of ACE genotypes accordingto ethnic groups have revealed various results and have caused controversy. METHODS: Polymerase chain reactions were performed to determine the ACE genotypes in fifteen formalinfixed, paraffin-embedded tissues from patients with sarcoidosis. RESULTS: The distribution of ACE gene (I/D) polymorphism in patients with sarcoidosis was significantly different from that in normal controls. The DD genotype was more frequent in patients with sarcoidosis than in thenormal controls. The D allele frequency was also higher in patients with sarcoidosis than in thenormal controls. The relative risk of sarcoidosis was higher in DD homozygotes. CONCLUSIONS: These results suggested the ACE gene I/D polymorphism may play an important rolein the pathogenesis and progression of sarcoidosis.
