IL-12 and IL-23 Production in Toxoplasma gondii- or LPS-Treated Jurkat T Cells via PI3K and MAPK Signaling Pathways.
10.3347/kjp.2017.55.6.613
- Author:
Hassan Ahmed Hassan Ahmed ISMAIL
1
;
Byung Hun KANG
;
Jae Su KIM
;
Jae Hyung LEE
;
In Wook CHOI
;
Guang Ho CHA
;
Jae Min YUK
;
Young Ha LEE
Author Information
1. Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 34134, Korea. yhalee@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Toxoplasma gondii;
LPS;
IL-12;
IL-23;
PI3K/AKT;
MAPK pathway
- MeSH:
Cytokines;
Humans;
Interleukin-12*;
Interleukin-23*;
Jurkat Cells;
p38 Mitogen-Activated Protein Kinases;
Phosphorylation;
T-Lymphocytes*;
Toxoplasma*
- From:The Korean Journal of Parasitology
2017;55(6):613-622
- CountryRepublic of Korea
- Language:English
-
Abstract:
IL-12 and IL-23 are closely related in structure, and have been shown to play crucial roles in regulation of immune responses. However, little is known about the regulation of these cytokines in T cells. Here, we investigated the roles of PI3K and MAPK pathways in IL-12 and IL-23 production in human Jurkat T cells in response to Toxoplasma gondii and LPS. IL-12 and IL-23 production was significantly increased in T cells after stimulation with T. gondii or LPS. T. gondii and LPS increased the phosphorylation of AKT, ERK1/2, p38 MAPK, and JNK1/2 in T cells from 10 min post-stimulation, and peaked at 30–60 min. Inhibition of the PI3K pathway reduced IL-12 and IL-23 production in T. gondii-infected cells, but increased in LPS-stimulated cells. IL-12 and IL-23 production was significantly reduced by ERK1/2 and p38 MAPK inhibitors in T. gondii- and LPS-stimulated cells, but not in cells treated with a JNK1/2 inhibitor. Collectively, IL-12 and IL-23 production was positively regulated by PI3K and JNK1/2 in T. gondii-infected Jurkat cells, but negatively regulated in LPS-stimulated cells. And ERK1/2 and p38 MAPK positively regulated IL-12 and IL-23 production in Jurkat T cells. These data indicate that T. gondii and LPS induced IL-12 and IL-23 production in Jurkat T cells through the regulation of the PI3K and MAPK pathways; however, the mechanism underlying the stimulation of IL-12 and IL-23 production by T. gondii in Jurkat T cells is different from that of LPS.
