Effect of Retinoic Acid on Growth and Transduced Tumor Necrosis Factor-alpha Gene Expression of Human Bladder Tumor Cell Lines.
- Author:
Hyeon JEONG
1
;
Sang Jin YOON
;
Moon Ki JO
;
Hae Won LEE
;
Soo Woong KIM
;
Eun Sik LEE
;
Chong Wook LEE
Author Information
1. Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
gene therapy;
tumor necrosis factor-alpha (TNF-alpha)
- MeSH:
Cell Line*;
DNA, Complementary;
Enzyme-Linked Immunosorbent Assay;
Gene Expression*;
Genetic Therapy;
Humans*;
Moloney murine leukemia virus;
Parents;
Terminal Repeat Sequences;
Tretinoin*;
Tumor Necrosis Factor-alpha*;
Up-Regulation;
Urinary Bladder Neoplasms*;
Urinary Bladder*;
Zidovudine
- From:Korean Journal of Urology
1997;38(3):229-234
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION AND OBJECTIVES: Retinoic acid (RA) is known as a potent chemopreventive agent in bladder tumor. Recently, RA has gained attention for up-regulation of transduced gene expression via long terminal repeat (LTR) transcriptional promotion. In this study, we investigated the possible dual effect of RA, growth inhibition and up-regulation of transduced gene expression which contains LTR promoter in human bladder carcinoma cell lines. MATERIALS AND METHODS: Human bladder carcinoma cell lines CY-24, J-82, HT-1197, ATCC) were transduced with Moloney murine leukemia virus containing cDNA of TNF-alpha. The growth of transduced and parent cell line was measured by tetrazolium based colorimetric assay (MTF). Transduced TNF-alpha gene expression was determined by ELISA method. RESULTS: TNF-alpha production was increased approximately twofold after treatment with RA (10 uM) in all three cell lines. This increase was dependent on RA concentration. RA treatment of transduced and parent cell line resulted in dose dependent inhibition of cell proliferation(up to 80% inhibitionwith 10 uM RA) in all parental and transduced cell lines. CONCLUSIONS: These results indicate that RA shows dual effect in cytokine gene transduced bladder carcinoma cells with retroviral vector containing LTR promoter and could be a supplement to the gene therapy of bladder cancer.
