Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax.
10.3347/kjp.2017.55.6.623
- Author:
Nguyen Thi Phuong LINH
1
;
Hyun PARK
;
Jinyoung LEE
;
Dong Xu LIU
;
Ga Eun SEO
;
Hae Jin SOHN
;
Jin Hee HAN
;
Eun Taek HAN
;
Ho Joon SHIN
;
Seon Ju YEO
Author Information
1. Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, Korea. yeosj@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Plasmodium vivax;
Plasmodium lactate dehydrogenase;
monoclonal antibody
- MeSH:
Antibodies;
Antibodies, Monoclonal*;
Diagnosis*;
Enzyme-Linked Immunosorbent Assay;
Fluorescent Antibody Technique;
Humans;
Immunoassay;
L-Lactate Dehydrogenase;
Limit of Detection;
Plasmodium vivax*;
Plasmodium*
- From:The Korean Journal of Parasitology
2017;55(6):623-630
- CountryRepublic of Korea
- Language:English
-
Abstract:
Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.
