Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway.
10.4142/jvs.2010.11.1.43
- Author:
Sunhee SHIN
;
Seong Soo JOO
;
Dongsun PARK
;
Jeong Hee JEON
;
Tae Kyun KIM
;
Jeong Seon KIM
;
Sung Kyeong PARK
;
Bang Yeon HWANG
;
Yun Bae KIM
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Angelica gigas;
croton oil;
cyclooxygenase-II;
inflammation;
prostaglandin E2
- MeSH:
Angelica/*immunology;
Animals;
Cell Line;
Cyclooxygenase 1/genetics/*immunology;
Cyclooxygenase 2/genetics/*immunology;
Dinoprostone/genetics/immunology;
Inflammation/drug therapy/enzymology/*immunology;
Interleukin-6/blood;
Macrophages;
Male;
Mice;
Mice, Inbred ICR;
Nitric Oxide/blood;
Phytotherapy/*methods;
Plant Extracts/*pharmacology/therapeutic use;
Plant Roots/immunology;
RNA, Messenger/chemistry/genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Necrosis Factor-alpha/blood
- From:Journal of Veterinary Science
2010;11(1):43-50
- CountryRepublic of Korea
- Language:English
-
Abstract:
The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG) were investigated in vitro and in vivo using croton oil-induced inflammation models. Croton oil (20 microgram/mL) up-regulated mRNA expression of cyclooxygenase (COX)-I and COX-II in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations of prostaglandin E2 (PGE2). EAG (1~10 microgram/mL) markedly suppressed croton oil-induced COX-II mRNA expression and PGE2 production. Application of croton oil (5% in acetone) to mouse ears caused severe local erythema, edema and vascular leakage, which were significantly attenuated by oral pre-treatment with EAG (50~500 mg/kg). Croton oil dramatically increased blood levels of interleukin (IL)-6 and PGE2 without affecting tumor-necrosis factor (TNF)-alpha and nitric oxide (NO) levels. EAG pre-treatment remarkably lowered IL-6 and PGE2, but did not alter TNF-alpha or NO concentrations. These results indicate that EAG attenuates inflammatory responses in part by blocking the COX-PGE2 pathway. Therefore, EAG could be a promising candidate for the treatment of inflammatory diseases.
