A Study on the Calcium-Binding Protein in Gallbladder Bile.
- Author:
Myung Hwan KIM
1
;
Sung Ae JUNG
;
Hee Bok CHAI
;
Hae Kyung KIM
;
Tae Hyung KIM
;
Byeong Moo YOO
;
Dong Wan SEO
;
Mee Hwa LEE
;
Sung Koo LEE
;
Hwoon Yong JUNG
;
Young Il MIN
Author Information
1. Department of Internal Medicine, Gallstone Study Group, Asan Medical Center, University of Ulsan, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Calcium-binding protein;
Bile;
Gallstones
- MeSH:
Autoradiography;
Bile*;
Calcium;
Cholesterol;
Electrophoresis, Polyacrylamide Gel;
Gallbladder*;
Gallstones;
Humans;
Salts
- From:Korean Journal of Medicine
1997;52(4):470-475
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: Calcium salts are usually present in the center of all types of gallstones, including pure cholesterol stones. It has been postulated that precipitation of calcium salts might act as a nidus for gallstone formation. Our goal was to determine whether a calcium-binding protein was present in bile and whether this protein has any calcium-binding properties. METHODS: Calcium-binding moiety was obtained by addition of CaC12 into the gallbladder bile. Calcium-binding protein was identified by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The capacity to bind calcium was confirmed by autoradiography with 45Ca++. RESULTS: We identified a protein(M.W.
