Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells.
10.4062/biomolther.2016.069
- Author:
Cheng Wen YAO
1
;
Kyoung Ah KANG
;
Mei Jing PIAO
;
Yea Seong RYU
;
Pattage Madushan Dilhara Jayatissa FERNANDO
;
Min Chang OH
;
Jeong Eon PARK
;
Kristina SHILNIKOVA
;
Soo Young NA
;
Seung Uk JEONG
;
Sun Jin BOO
;
Jin Won HYUN
Author Information
1. School of Medicine No.1 and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea. sunjinboo@jejunu.ac.kr
- Publication Type:Original Article
- Keywords:
Autophagy;
5-Fluorouracil;
SNUC5/5-FUR;
Reactive oxygen species;
Colon cancer
- MeSH:
Acridine Orange;
Autophagy*;
Colon*;
Colonic Neoplasms*;
Cysteine;
Flow Cytometry;
Fluorouracil*;
Microscopy;
Microscopy, Confocal;
Reactive Oxygen Species
- From:Biomolecules & Therapeutics
2017;25(3):315-320
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.
