Capsanthin Inhibits both Adipogenesis in 3T3-L1 Preadipocytes and Weight Gain in High-Fat Diet-Induced Obese Mice.
10.4062/biomolther.2017.048
- Author:
Sung Jun JO
1
;
Jeung Won KIM
;
Hye Ok CHOI
;
Jung Hwan KIM
;
Hyung Joong KIM
;
Sun Hee WOO
;
Byung Hoon HAN
Author Information
1. Life Science Research Institute, E.S. Biotech. Co. Ltd., Cheonan 31257, Republic of Korea. bhhan3312@yahoo.co.kr
- Publication Type:Original Article
- Keywords:
Capsanthin;
Anti-adipogenic;
Lypolytic activity;
Spontaneous locomotive activity;
Adiponectin
- MeSH:
Adenosine Triphosphate;
Adipocytes;
Adipogenesis*;
Adiponectin;
Animals;
Diet, High-Fat;
Fatty Acids;
Insulin;
Mice;
Mice, Obese*;
Models, Animal;
Weight Gain*
- From:Biomolecules & Therapeutics
2017;25(3):329-336
- CountryRepublic of Korea
- Language:English
-
Abstract:
Adipogenesis in murine preadipocyte 3T3L-1 has been used as a model system to study anti-obese bioactive molecules. During adipogenesis in 3T3-L1 preadipocytes, we found that capsanthin inhibited adipogenesis (IC₅₀; 2.5 μM) and also showed lipolytic activity in differentiated adipocytes from the preadipocytes (ED₅₀ ; 872 nM). We identified that the pharmacological activity of capsanthin on adipogenesis in 3T3-L1 was mainly due to its adrenoceptor-β2-agonistic activity. In high-fat diet animal model study, capsanthin significantly enhanced spontaneous locomotive activities together with progressive weight-loss. The capsanthin-induced activation of kinetic behavior in mice was associated with the excessive production of ATP initiated by both the enhanced lipolytic activity together with accelerated oxidation of fatty acids due to the adrenoceptor β2-agonistic activity of capsanthin. Capsanthin also dose-dependently increased adiponectin and p-AMPK activity in high fat diet animals, suggesting that capsanthin has both anti-obesity and insulin sensitizing activities.
