Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation.
- Author:
Myung Jo YOU
1
Author Information
1. College of Veterinary Medicine and Bio-safety Research Center, Chonbuk National University, Jeonju 561-756, Korea. tick@chonbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
ticks;
troponin I;
antigen;
immunity;
biological control
- MeSH:
Animals;
Feeding Behavior;
Female;
Ixodidae/*immunology;
Mice;
Mice, Inbred BALB C;
Microfilament Proteins/*immunology;
Recombinant Proteins/immunology;
Tick Infestations/*prevention&control;
Vaccines, Synthetic/immunology
- From:Journal of Veterinary Science
2005;6(1):47-51
- CountryRepublic of Korea
- Language:English
-
Abstract:
The success of immunological control methods is dependent upon the use of potential key antigens as tick vaccine candidates. Previously, we cloned a gene encoding 27 kDa and 30 kDa proteins (P27/30) of Haemaphysalis longicornis, and identified the P27/30 is a troponin I-like protein. In this study, the recombinant P27/30 (rP27/30) expressed in Escherichia coli was used to immunize mice and the mice were challenge-infested with ticks at different developmental stages of the same species. The rP27/30 protein stimulated a specific protective anti-tick immune response in mice, evidenced by the statistically significant longer pre-feeding periods in adult ticks. Furthermore, significantly longer feeding periods were noted in both larval and adult ticks. On the other hand, only larval ticks exhibited low attachment rates (31.1%). Immunization of mice with rP27/30 protein confers protection against hard tick Haemaphysalis longicornis infestation. These results demonstrated that the rP27/30 protein might be a useful vaccine candidate antigen for biological control of ticks.
