Comparative Study on Development of Mouse Embryos in Three Commercial Media and Hatching Rates of Mouse Embryos with/without Pronase.
- Author:
Jeong Heon LEE
;
Hee Jeong GO
;
Geu Jeong CHAE
;
Ki Suk LEE
;
Jong Duk KIM
- Publication Type:Comparative Study ; Original Article
- Keywords:
Mouse;
Media;
Pronase
- MeSH:
Animals;
Digestion;
Embryonic Development;
Embryonic Structures*;
Female;
Mental Competency;
Mice*;
Morula;
Pregnancy;
Pregnancy Rate;
Pronase*;
Zona Pellucida
- From:Korean Journal of Fertility and Sterility
2001;28(3):235-246
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The purpose of this present study was to compare mouse embryo development in 3 commercial media and hatching competence of mouse embryo with or without enzymatic treatment. METHODS: Collected 375 mouse embryos were divided into three groups, and then cultured in IVF-20 (G2), Medicult IVF (M3), P-1 (blastocyst M), respectively. Three day mouse morulae were cultured in G2 media treated with pronase. The results were analyzed using Chi-square test, and considered statistically significant when p<0.01. RESULTS: The developmental rate of 2 cell mouse embryo after 72 hours was highest in IVF-20 (G2) among conventional 3 media. The hatching rate of mouse morulae was low when clultured in G2 media without pronase during 48 hours. However, it was higher when cultured in media treated with l mg/ml, 2.5 mg/ml, 5 mg/ml pronase, respectively. CONCLUSIONS: Using good media and digestion of zona pellucida with enzymatic treatment improve development and hatching rate of embryo. Therefore, implantation and pregnancy rate could be improved.
