Gene expression profiling of mouse aborted uterus induced by lipopolysac charide.
- Author:
Jeong Mi MOON
1
;
Song Eun LEE
;
Yong Il MIN
;
Chaeyong JUNG
;
Kyu Youn AHN
;
Kwang Il NAM
Author Information
- Publication Type:Original Article
- Keywords: Abortion; Lipopolysaccharide; Microarray; Mouse
- MeSH: Animals; Cytoskeleton; Extracellular Matrix; Gene Expression; Gene Expression Profiling; Mice; Oligonucleotide Array Sequence Analysis; Pregnancy; RNA; Toll-Like Receptors; Uterus
- From:Anatomy & Cell Biology 2011;44(2):98-105
- CountryRepublic of Korea
- Language:English
- Abstract: To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
