Evaluation of bone formation by recombinant human BMP-2 and rapid prototype titanium cap in rabbit calvaria using micro computed tomography.
10.5125/jkaoms.2010.36.6.466
- Author:
Man Seung HAN
1
;
Seunggon JUNG
;
Bang Sin KIM
;
Ji Woong YANG
;
Min Suk KOOK
;
Hong Ju PARK
;
Sun Youl RYU
;
Hee Kyun OH
Author Information
1. Department of Oral and Maxillofacial Surgery, 2nd stage of Brain Korea 21 (BK21), School of Dentistry, Chonnam National University, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. hkoh@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Titanium cap;
Bone morphogenetic protein 2;
Micro computed tomography
- MeSH:
Animals;
Bone Morphogenetic Protein 2;
Bone Morphogenetic Proteins;
Calcium Phosphates;
Humans;
Osteogenesis;
Rabbits;
Skull;
Titanium;
Transplants
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2010;36(6):466-472
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
INTRODUCTION: This study examined the effect of recombinant human bone morphogenetic protein (rhBMP)-2 and beta-tricalcium phosphate (beta-TCP) on new bone formation in a rabbit calvarium using a rapid prototype titanium cap (RP Ti cap). MATERIALS AND METHODS: Eight New Zealand white rabbits were used in this study. Hemispherical RP Ti caps (10 mm in diameter) were implanted subperiosteally on the rabbit calvaria. beta-TCP was filled in the RP Ti cap in the control group, and rhBMP-2 soaked beta-TCP was used in experimental group. The rabbits were sacrificed 2 and 4 weeks after the operation. The volume and pattern of newly formed bone was analyzed by micro computed tomography (CT). RESULTS: Macroscopically, there were no abnormal findings in any of the animals. The micro CT images revealed new bone from the calvaria that expanded gradually toward the top of the titanium cap, particularly along the inner surface of the titanium cap in the experimental group at 4 weeks after grafting. There was no significant difference in new bone volume ratio between the control and experimental groups at 2 weeks after grafting. There was a statistically significant difference in the new bone volume ratio between the experimental (14.1+/-1.8 %) and control (7.2+/-1.5 %) groups at 4 weeks after grafting (P<0.01). CONCLUSION: The RP Ti cap can effectively guide new bone formation and rhBMP-2 can induce the new bone formation.
