Change of expression of IL-4, IL-5, and IFN-r mRNA in Der p I-specific T-cell clones.
- Author:
Sang Heon CHO
;
Soo Jong HONG
;
Yoon Keun KIM
;
Jae Kyung PARK
;
Jung Yeon SHIM
;
Hee Bom MOON
;
Kyoun Up MIN
;
You Young KIM
- Publication Type:Original Article
- Keywords:
IL-4;
IL-S;
IFN-y;
Der pI;
asthma
- MeSH:
Asthma;
Clone Cells*;
Humans;
Immunotherapy;
Interleukin-4*;
Interleukin-5*;
Lymphocytes;
RNA, Messenger*;
T-Lymphocytes*
- From:Journal of Asthma, Allergy and Clinical Immunology
1998;18(4):720-727
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: It is known that immunotherapy promotes the development of allergen-specific Thl-like lymphocytes whose products are effective in inhibiting clinical response of sensitized atopic patients to allergen exposure. At the single cell level in short term culture, however, IL-4 and IL-5 are co-expressed, while IL-4 and IFN-y are exclusively expressed. OBJECTIVE: IL-4, IL-5, and IFN-y mRNA were measured in Der pI-specific T-cell clones (TCCs) to evaluate whether expression of cytokine in allergen-specific TCC is fixed regardless of stimuli. METHOD: Seven Der pI-specific TCCs were made from two asthmatics sensitive to D. pteronyssinus. IL-4, IL-5, and IFN-y mRNA were measured by RT-PCR in these TCCs after antigen-specific (Der pI) and nonspecific (PHA + TPA) stimuli. RESULTS: IL-4 and IL-5 mRNA were expressed in four and six of seven TCCs, but IFN-y mRNA was not expressed in any TCCs after Der pI-specific stimuli. Meanwhile, after the stimulus of TPA plus PHA, IFN-y mRNA as well as IL-4 and IL-5 mRNA were expressed in four of seven TCCs, and in one TCC, only IFN-y mRNA was expressed without expression of IL-4 mRNA. CONCLUSION: The expression of cytokine may be variable in allergen-specific TCC according to the type and amount of stimuli.
