Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells.
- Author:
Niti PURI
1
;
R K SAXENA
Author Information
1. School of Life Sciences Jawaharlal Nehru University, New Delhi, India.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
B cells;
proliferation;
cytokines;
LPS;
growth factor
- MeSH:
Animal;
B-Lymphocytes/physiology*;
B-Lymphocytes/drug effects;
Bone Marrow/metabolism;
Cell Division/physiology;
Chromatography, High Pressure Liquid;
Chymotrypsin/pharmacology;
Dose-Response Relationship, Drug;
Growth Substances/pharmacology*;
Growth Substances/chemistry;
Heat;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Molecular Weight;
Polymyxin B/pharmacology;
Protein Denaturation;
Spleen/metabolism*;
Thymus Gland/metabolism;
Trypsin/pharmacology
- From:Experimental & Molecular Medicine
1998;30(4):199-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
