Genomic structure of the regulatory region of the voltage-gated calcium channel alpha 1D.
- Author:
Hyung Lae KIM
1
;
Yoon Jeong CHANG
;
Sang Moo LEE
;
Young Sook HONG
Author Information
1. Department of Biochemistry, College of Medicine, Ewha Womans University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
calcium channel;
gene expression;
regulation;
molecular cloning
- MeSH:
Amino Acid Sequence;
Base Sequence;
Calcium Channels/genetics*;
Chloramphenicol O-Acetyltransferase/genetics;
Cloning, Molecular;
Gene Library;
Genetic Vectors;
Human;
Molecular Sequence Data;
Regulatory Sequences, Nucleic Acid*;
Restriction Mapping
- From:Experimental & Molecular Medicine
1998;30(4):246-251
- CountryRepublic of Korea
- Language:English
-
Abstract:
In excitable and endocrine organs, calcium influxes through the L-type voltage-gated calcium channel (VGCC) which is composed of four (alpha 1, alpha 2, beta, and gamma) subunits. Temporal and spatial expression of calcium channel activity is regulated by the transcription of alpha 1 subunit. To elucidate the genomic organization of the VGCC alpha 1D subunit gene, a genomic clone was isolated from the human genomic library and its sequence was analyzed. A 12 kb genomic clone contained the 5'-flanking regulatory region and first two exons was selected and the initiation site for alpha 1D mRNA synthesis was examined by primer extension analysis. The major initiation site was found at the -523 NT position in the translation initiation site. The TATA box could not be found above the transcription initiation site. The CAT vector construct containing the 2.5 kb upstream region had high CAT activity on transfection to NG108-15 and PC12 cells, which confers the neuronal expression of the alpha 1D gene.
