Protective effects of a transgenic carrot vaccine on piglet diarrhea.
- Author:
Yeong Hun KIM
1
;
Jin Young NAM
;
Hyang Keun LEE
;
Cheol Ho HWANG
;
Jeong Hee HAN
Author Information
1. College of Veterinary Medicine and Institution of Veterinary Science, Kangwon National University, Chuncheon 200-701, Korea. hanjh@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
Enterotoxigenic Escherichia coli K88ac;
maternal antibody;
plant edible vaccine
- MeSH:
Ants;
Colostrum;
Daucus carota;
Diarrhea;
Duodenum;
Enterotoxigenic Escherichia coli;
Female;
Ileum;
Immunoglobulin A;
Immunoglobulin G;
Jejunum;
Korea;
Lactation;
Vaccines
- From:Korean Journal of Veterinary Research
2011;51(2):151-158
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The study evaluated whether a transgenic carrot vaccine could induce a K88-specific immune response in sows and whether the resultant maternal antibody could protect piglets against enterotoxigenic Escherichia coli (ETEC) K88ac infection. Sows (n = 15) selected randomly from a farm in Korea were assigned to three groups (n = 5 per group: control [untreated]), group A (orally inoculated with a non-transgenic and transgenic carrot vaccines at 2 and 4 weeks ante partum, respectively), and group B (conventionally vaccinated according to the manufacturer's instructions). After 7 days of lactation, 5 piglets selected randomly from each group were challenged with 1 x 1010 colony forming units/mL ETEC K88ac. Group C had the lowest mean fecal consistency score on post-challenge days 1 and 7. Histiologically, On post-challenge day 7, group C showed an increased duodenum and ileum villus:crypt ratio, compared to group A in the duodenum, with group B displaying the highest ratio. Groups B and C had more increased villus width than group A in the jejunum. Group C displayed the greatest increase in villus width in the ileum. The colostrums and serum from groups B and C displayed higher concentrations of IgA and IgG against ETEC K88, compared to group A. Based on the results, it was concluded that the transgenic carrot vaccine in sow per oral may have an effect on preventing piglet diarrhea as good as commercial recombinant vaccine.
