Anti-inflammatory and Anti-tumorigenic Effects of Açai Berry in Helicobacter felis-infected mice.
10.15430/JCP.2016.21.1.48
- Author:
Ju Yup LEE
1
;
Nayoung KIM
;
Yoon Jeong CHOI
;
Ryoung Hee NAM
;
Seonmin LEE
;
Min Hee HAM
;
Ji Hyung SUH
;
Yoon Jin CHOI
;
Hye Seung LEE
;
Dong Ho LEE
Author Information
1. Departments of Internal Medicine, Keimyung University School of Medicine, Daegu, Korea. nayoungkim49@empas.com
- Publication Type:Original Article
- Keywords:
Açai (Euterpe oleracea);
Stomach;
Helicobacter felis;
Inflammation
- MeSH:
Animals;
Atrophy;
Carcinogenesis;
Cats;
Colon;
Diet;
Enzyme-Linked Immunosorbent Assay;
Felis;
Female;
Fruit*;
Helicobacter felis;
Helicobacter*;
Humans;
Inflammation;
Metaplasia;
Mice*;
Monocytes;
Neutrophils;
Peroxidase;
Stomach
- From:Journal of Cancer Prevention
2016;21(1):48-54
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: The aim of this study was to evaluate the anti-inflammatory and anti-tumorigenic effect of açai berry after chronic Helicobacter felis colonization in the stomachs of C57BL/6 mice. METHODS: A total of 57 four-week-old female C57BL/6 mice (18 control mice and 39 experimental mice) were used. The mice were administered orogastrically with vehicle only or vehicle containing H. felis, 5 times every other day. After inoculation of H. felis, mice were fed either a standard or an açai-containing diet and then sacrificed at 4, 24, and 52 weeks. The infection status and degree of inflammation were determined by culture and histopathology. The level of gastric mucosal myeloperoxidase (MPO), TNF-α, and interleukin-1β (IL-1β) were measured by ELISA. RESULTS: At 24 weeks after inoculation, mucosal atrophy and mucous metaplasia appeared in all infected mice. At 52 weeks after inoculation, dysplastic change was noted in 10%, 25%, and 50% of mice in the H. felis-control, H. felis-açai 5%, and H. felis-açai 10% groups, respectively. The neutrophil, monocyte, atrophy, and metaplasia grades of infected mice showed no significant difference among the H. felis-infected groups. H. felis-infected mice fed with açai berry showed no significant difference compared with H. felis-infected control mice in gastric mucosal MPO, TNF-α, and IL-1β levels. CONCLUSIONS: H. felis that colonized the stomachs of C57BL/6 mice provoked inflammation, and induced mucosal atrophy, metaplasia, and dysplasia. However, açai berry did not effectively prohibit the gastric carcinogenesis which was induced by chronic H. felis infection.
