Development of Porcine Pericardial Heterograft for Clinical Application(Tensile Strength-thickness).
- Author:
Kwan Chang KIM
1
;
Cheul LEE
;
Chang Hue CHOI
;
Chang Ha LEE
;
Sam Sae OH
;
Seong Sik PARK
;
Kyung Hwan KIM
;
Woong Han KIM
;
Yong Jin KIM
Author Information
1. Department of Thoracic and Cardiovasular Surgery, Ewha Womans University, Korea.
- Publication Type:Original Article
- Keywords:
Calcification;
Heterograft;
Glutaraldehyde
- MeSH:
Bioprosthesis;
Cardiovascular Diseases;
Ethanol;
Glutaral;
Handling (Psychology);
Humans;
Pericardium;
Sodium;
Tensile Strength;
Transplantation, Heterologous;
Transplantation, Homologous
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2008;41(2):170-176
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Bioprosthetic devices for treating cardiovascular diseases and defects may provide alternatives to autologous and homograft tissue. We evaluated the mechanical and physical conditions of a porcine pericardial bioprosthesis treated with Glutaraldehyde (GA), Ethanol, or Sodium dodecylsulfate (SDS) before implantation. MATERIAL AND METHOD: 1) Thirty square-shaped pieces of porcine pericardium were fixed in 0.625%, 1.5% or 3% GA solution. 2) The tensile strength and thickness of these and other bioprosthesis, including fresh porcine pericardium, fresh human pericardium, and commercially produced heterografts, were measured. 3) The tensile strength and thickness of the six treated groups (GA-Ethanol, Ethanol-GA, SDS only, SDS-GA, Ethanol-SDS-GA and SDS-Ethanol-GA) were measured. RESULT: 1) Porcine pericardium fixed in 0.625% GA the thinnest and had the lowest tensile strength, with thickness and tensile strength increasing with the concentration of GA solution. The relationship between tensile strength and thickness of porcine pericardium increased at thicknesses greater than 0.1 mm (correlation-coefficient 0.514, 0<0.001). 2) There were no differences in tensile strength or thickness between commercially-produced heterografts. 3) Treatment of GA, ethanol, or SDS minimally influenced thickness and tensile strength of porcine pericardium, except for SDS alone. CONCLUSION: Porcine pericardial bioprosthesis greater than 0.1 mm thick provide better handling and advantageous tensile strength. GA fixation did not cause physical or mechanical damage during anticalcification or decellularization treatment, but combining SDS-ethanol pre-treatment and GA fixation provided the best tensile strength and thickness.
